Journal: STAR Protocols

Article Title: Protocol for validating computationally predicted splice-altering variants using full-length gene reporter assays

doi: 10.1016/j.xpro.2026.104433

Figure Lengend Snippet: Transient splicing reporter assay using the genomic locus sequence reproduces the endogenous TREM2 and APOE isoform patterns (A) Schematic representation of the genomic TREM2 and APOE sequences cloned into the pcDNA3.1(+) vector. (B) Human cell lines expressing endogenous TREM2 (THP-1, HMC3), non-expressing HEK293T cells, and mouse microglial BV2 cells were transfected with the full-length (FL) TREM2 reporter. (C) HMC3 and HEK293T cells expressing endogenous APOE were transfected with the FL APOE reporter. HB, endogenous TREM2 (B) and APOE (C) transcripts from human brain (frontal cortex). cDNA synthesis was primed with either oligo(dT) or a plasmid-specific RT primer (P) positioned downstream of the gene insert. Arrows mark RT-PCR primer positions. Amplified products were resolved by agarose gel electrophoresis.

Article Snippet: HEK293T , ATCC , CRL-3216.

Techniques: Reporter Assay, Sequencing, Clone Assay, Plasmid Preparation, Expressing, Transfection, cDNA Synthesis, Reverse Transcription Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis

Journal: Molecules

Article Title: Sacha Inchi ( Plukenetia volubilis L.) Oil Press-Cake Powder: Chemical Characterization and In Vitro Bioactivity for Sustainable Applications

doi: 10.3390/molecules31010117

Figure Lengend Snippet: Effect of SICP Extract on Cell Viability in Different Cell Lines. Cell viability was assessed using the MTT assay following 72 h of incubation with SICP extract (SIPC, dilution 1:80) in different human cell lines: ( A ) HEK (human embryonic kidney, non-tumoral control), ( B ) Panc-1 (pancreatic ductal adenocarcinoma), ( C ) HepG2 (hepatocellular carcinoma), and ( D ) PC-3 (prostate adenocarcinoma). NT indicates untreated control cells; S1 indicates cells treated with the solvent mixture alone (acetone/water/acetic acid, 80/19/1 v / v / v ), and SIPC indicates cells treated with SICP extracts. Results are expressed as percentage of cell viability relative to untreated controls (NT, 100% viability). Each bar represents the mean ± standard error of the mean (SEM), and the number inside each bar indicates the number of independent biological replicates performed. Statistical significance was evaluated by one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05 vs. NT control).

Article Snippet: Panc-1 (human pancreatic ductal adenocarcinoma), HepG2 (human hepatocellular carcinoma), Calu-3 (human lung adenocarcinoma), PC-3 (human prostate adenocarcinoma), and HEK (human embryonic kidney) cell lines were maintained in appropriate growth media following ATCC recommendations.

Techniques: MTT Assay, Incubation, Control, Solvent